ALU pcr lab
Purpose- Successfully isolate DNA from cheek cells and prepare a PCR reaction for amplification of an Alu insert.
Materials-
.9% saline solution
micropipette tips
waste container
microcentrifuge
microcentrifuge tubes
PCR tubes
agarose
1x TAE
gel chambers + molds
load dye
chelex
racks
primer mix
master mix
H20
+ control DNA
Procedure- We started by getting DNA from inside our mouths by swishing salt water around and put that into a microfuge tube, then spinning that. We got rid of all but the cell pellet at the bottom. Then we added saline to resuspend the cells and also some 5% chelex in. We then put our tube into a heat block for 10 minutes. After spinning, we took out a portion of the top part of the liquid, not the chelex beads at the bottom into a new tube. We took master mix and put it into the small PCR tube, also adding primer mix and a smaller amount of DNA. After a spin, we pipetted our mix into the agarose gel. After the electrophoresis was completed it will be photographed.
Materials-
.9% saline solution
micropipette tips
waste container
microcentrifuge
microcentrifuge tubes
PCR tubes
agarose
1x TAE
gel chambers + molds
load dye
chelex
racks
primer mix
master mix
H20
+ control DNA
Procedure- We started by getting DNA from inside our mouths by swishing salt water around and put that into a microfuge tube, then spinning that. We got rid of all but the cell pellet at the bottom. Then we added saline to resuspend the cells and also some 5% chelex in. We then put our tube into a heat block for 10 minutes. After spinning, we took out a portion of the top part of the liquid, not the chelex beads at the bottom into a new tube. We took master mix and put it into the small PCR tube, also adding primer mix and a smaller amount of DNA. After a spin, we pipetted our mix into the agarose gel. After the electrophoresis was completed it will be photographed.